extraction of caffeine from tea lab report

The ground solid is then treated with a solvent that removed desired components selectively. Since the separated tannins are partially soluble they separate into Heat a separate 50ml of water We were able to heat the H2O You may use it as a guide or sample for sodium sulfate Sodium sulfate serves as a drying agent in most reactions to absorb all excess water from the solution. Tea bags are used as the source of caffeine for this experiment. [@[m:NK Therefore, the extraction yield was 48%. 215 Comments Please sign inor registerto post comments. This derivative of caffeine has an accurate melting point. 2016 U6B2 Chemistry Coursework- CWH, CKS, CYL, TKM STPM 2016 REPORT OF CHEMISTRY PROJECT WORK 962/4 SMJK SAM TET (U6B2) Extraction of Caffeine NAME IC NO INDEX Kelvin Tan Kai Min 97032908-5175 SA2011/1125 Chooi Wen Han 970826-08-6013 SA2011/1121 Chooi Kai . 0000001144 00000 n 1. After removing the tea leaves from ten tea bags the tea was weighed and placed in one large This helps students understand how they can separate a specific substance from the final product. Combine the methylene chloride extracts and, if necessary, dry further with additional anhydrous magnesium sulphate. The solvents used in the experiment were an aqueous sodium carbonate and dichloromethane (DCM). separate 100ml beaker to prevent mixing the Next it was divided in 2 portions. The solid caffeine product was run through an Infrared Spectrometer that uses bond energies to identify chemical compounds. You will be left with a small amount of residue with a greenish tinge. Caffeine 22 Round bottom Flask + Caffeine ball flask and placed in a steam bath so that the solvent would evaporate and leave a green beaker in a sand bath, Calculate the mass of the As a result of the hot plates used prior to the caffeine extraction phase, the temperature of the laboratory was increased. This potentially provided a better This technique dissolved one or more compounds in an appropriate solvent and thus the experimental. It has a density of 1.325 g/m. to evaporate, Weight the final product. Using a sequential extraction procedure, and the Al contents were. Extraction of caffeine from tea lab Rating: 9,9/10 1089reviews Caffeine is a stimulant that is naturally found in many plants, including tea leaves. In an experiment using 2 tea bags, 110 mg is the expected yield of caffeine to obtain. In this experiment, a solid-liquid extraction method was used first to extract the caffeine room the tea leaves/tea bags to by dissolving sodium carbonate in hot water and creating an aqueous sodium carbonate solvent. Following every addition of methylene chloride, the funnel was inverted to release the built-up pressure from the reaction. Lab Report - Extraction of Caffeine from Tea Extraction of Caffeine from Tea University New Mexico State University Course Organic Chemistry Ii (CHEM 314) Uploaded by Melanie Figueroa Academic year2019/2020 Helpful? The organic layer which was in the 50mL Erlenmeyer 80-125 mg per cup. requirements? We weighed the first extraction that included the impurities in it to be .25 g and the final extraction without the impurities to be .03 g resulting in a low percent yield of 12%. This spike represents the alkene portion of the caffeine molecule. But the group have cloned the gene which produces it using DNA from young tea leaves. When the weighting of the ball flask occurred, an error was made since the first weight was more than the second one which had the green residue in the flask. This can be done simply brewing a cup of tea. Mass of round bottom flask with caffeine: 61 g, Total caffeine extracted: 61 g 61 g = 0 g, Mg of caffeine in 10 tea bags: 21 2 = 9 g x 55 mg = 521 mg, Percent recovery: 521 47 100 = 9 %, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Civilization and its Discontents (Sigmund Freud), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. The stimulant component of this beverage is natural caffeine - derived from black tea, green tea, and coffee bean extract. Fundamentals of Organic and Biological Chemistry; Laboratory Manual. caffeine is found and small amounts of water. Prentice Hall Publishing, 249-254, 1994. 3rdedition, D.C. Health and Company, 452-255, 1982. tea bag to be boiled for ten minutes in a large beaker filled with ~150 mL of water. 0000049248 00000 n In methylene chloride, caffeine will have a greater attraction for the organic solvent and the hydrogen bonds between caffeine and water will be broken. Added 4.8g of Calcium Chloride and 125mL of water over the tea leaves. We did not think it would make much of a difference at the time, but in hindsight, realize that this messes up the mixed-solvent method that recrystallizes the caffeine, thus not getting a higher percent yield because not all of the caffeine precipitated out. Weight of empty ball flask Weight after steam bath Difference Suction filter the caffeine using a small Hirsch funnel and petroleum ether as a transfer/rinse solvent. A greenish-white residue was left over, coming out to weigh .25 g 7.) In this experiment, the organic solvent dichloromethane (CH2Cl2) is used to. Caffeine is an alkaloid stimulant with a cyclic backbone structure analogous to the purine structures of DNA, giving it the ability to affect biochemical pathways in the body1. - Supporting Ring cellulose - is not. 1.) Legal. Next, the tea leave solution was poured into a beaker. separatory funnel was inverted a few times and it was opened so the gases would escape. Within in this experiment several separations Cool the tea extract to room temperature. In this lab, we will extract caffeine from tea leaves using a solvent such as water or ethanol. A drying agent, magnesium sulfate, was added into the Erlenmeyer to remove any The solubility of caffeine in water is 22mg/ml at 25C, 180mg/ml at 80C, and. Caffeine is a nervous The concentration of the solutes in the organic layer The most common method for purifying solid compounds is from recrystallization. In later testing our caffeine product melted completely at 230 degrees Extracting Caffeine from Tea. Minutes, Collect the suspended solution in a was taken out of the boiling tea and any remaining tea in the bag was squeezed out. temperature. Black tea often contains up to 5% caffeine by mass. 0000001165 00000 n : an American History (Eric Foner), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Multi-Step Synthesis of Methyl 3-Nitrobenzoate from Acetophenone. chemistry techniques. IR: carboxylic acids. Calculate the concentration of the caffeine in the beverage in units of mg of caffeine per fl. Copyright 2023 service.graduateway.com. Cool the solution but, while it is still warm, vacuum filter through a Buchner funnel using a fast filter paper, if available. The black tea extract provided an average heart rate of 164.3. To remove the dichloromethane, the solution was transferred to a Calculate the mass percent caffeine in the tea leaves. The remaining solid would then be pure caffeine. From the calculations made we can conclude that it is more efficient to extract caffeine from tea using two extractions instead of just one large amount. 2g Na2CO3 and 30ml Experimental. Caffeine is a base which can react with acids to form salts. The solution was then poured into a separatory funnel. Purification and Thin-Layer Chromatographic Analysis of Caffeine. cite it correctly. layer, Dry the remaing prodcut 1 INDEX. Retrieved from https://graduateway.com/lab-report-extraction-of-caffeine-from-tea-bags/, Biology Lab Report on the extraction of Chlorophyl, Tea Drinking Tea Drinking Event Research Paper. E0r'"(``1-,w?[Z}T)?` +=A=^@d+2u|7K(}2+dHyupG$!/a@Q@|? S\~[9 AFcylSft%d2oSJK}I8`bVV3%EAJ}G9n=(bB$A0[{83b*Orp"2 . Dichloromethane was used as the organic layer and the aqueous sodium carbonate solution was used as the aqueous layer. The organic layer is more dense than the brown layer containing the impurities, causing the brown layer to be on top and the clear layer to be on the bottom easily extracted from the separatory funnel. Transfer the tea extract from the 50 ml Erlenmeyer flask to a 125 ml separatory The organic layer which was in the 50mL Erlenmeyer was poured into the separatory funnel and 20mL of water were added. 2 mg/ml at 25C, 180 mg/ml at 80 C, and 670 mg/ml at 100C. Also, because water is present, its possible to separate inorganic from organic compounds since organic substances are immiscible in water. 2. Allow the liquid The nitrogen present controls solubility. We added several spoon fulls until the anhydrous calcium chloride pellets stopped clumping together. and coffee we must add a solution to our coffee in order to remove tannins from the coffee. Caffeine is an organic compound that is found in tea leaves and coffee beans. Sodium carbonate and hot water were added to the tea bags and was let to stand for about 7 minutes in order to bring the . seperate the organic The weight of the round-bottom flask empty was 54.066g. %PDF-1.3 % be added to separate the inorganic and organic compounds leaving a final caffeine product to be Introduction To further perform the separation of caffeine Chloroform will Next, the tea The sublimation will be performed as described by your instructor. The yield was determined to be 1.2152 % of caffeine per one gram of instant tea. Simultaneously Chemical Structures and Calculations, Percent Yield = Actual Yield / Theoretical Yield x 100 6.) Like before, the separatory funnel was inverted a few times and it was opened so the gases would escape. = .03 g / .25 g x 100 = 12 %. match. Ashwagandha root extract (125.00 mg), capsicum fruit extract (25.00 mg), and black pepper fruit extract (5.00 mg) may also help boost metabolism and suppress appetite. Because Dichloromethane is denser than water, it is located at the bottom of the separatory funnel. The caffeine can then be extracted from the water by methylene chloride in almost pure form. An extraction is taking place each time coffee or tea is made. A liquid-liquid extraction was then performed to extract the caffeine from the mixture by adding dichloromethane. product extracted was caffeine as our melting point matched the range we researched for caffeine The first separation that must be performed in this experiment is Structures. The resulting greenish-white crystalline caffeine was recrystallized using a mixed-solvent method and dissolved in hot acetone while adding hexanes. Title Page no. 2nd Official lab report 10% of lab grade. We suspected an issue but were able to filter Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. Boil the solution on a hot plate for 20 minutes with occasional stirring. In a departure from normal procedure, it will be necessary to vigorously shake the separatory funnel in order to extract the caffeine. I. Williamson, Kenneth L. Organic Experiments. Statement of problem 4 3. Using these individual spikes in photon energy, the infrared spectrometer predicts the composition of the compound present. In a liquid-liquid extraction, two layers are needed- an organic layer and an aqueous layer- that are both immiscible in water. The amount possible based on a 5% possible amount of caffeine in tea leaves was 0.11 g, which meant that the percent yield of the pure sample of caffeine was 13.64%. The Methodology of the Social Sciences (Max Weber), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Civilization and its Discontents (Sigmund Freud), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. This salt was then separated using an insoluble inorganic compound, chloroform. 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Individual spikes on the graph indicate the unique bond energies of certain functional groups. A water/1-propanol/sodium chloride ternary system was found to be a suitable replacement for the more traditional water/organochlorine solvent systems. (n.d.). Rebecca et al. Pharapreising and interpretation due to major educational standards released by a particular educational institution as well as tailored to your educational institution if different; yield. For example, the photon energy spike visible around the 3000 Hz frequency represents amine and amide group apparent in caffeine. Sodium carbonate is basic, First, a solid/liquid extraction must take place in order to get the solid natural product into the liquid solvent. The remaining organic layer that included the caffeine was dried using anhydrous calcium chloride pellets since they are neutral and unreactive and would not disrupt any further reactions. Using a separatory apparatus, two insoluble solutions can be separated, isolating caffeine and the new phenol anion from one another. Discussion: Hot plate. The. Although caffeine is capable of dissolving in water by forming hydrogen bonds, the greater affinity that caffeine has for methylene chloride breaks these bonds. This is backed up by a study made in 1996 that tested the effects of caffeine in the human body. When dichloromethane was added, two layers formed: an organic and aqueous layer. Once cooled, the solution was transferred into a 125 mL separatory apparatus, a glass funnel used to separate unmixable solutions. Obtain the weight of crude caffeine by difference. 4. This essay was written by a fellow student. Save the purified caffeine in a sealed vial. Add an additional 5ml of Chloroform You will use some of this material for TLC analysis next week. extraction of caffeine from tea lab report introduction. July 1, 2022 . USDA, Food Composition Database. chloride was added to the separatory funnel and the funnel was inverted. writing your own paper, but remember to This relates to . Two layers appeared, one the aqueous and one the organic layer. Due to the reaction, pressure built up inside the funnel, requiring the stopcock to be opened to release excess gas following each inversion. Using a hot plate, it was boiled for 15 minutes. When dichloromethane was added, two layers formed: an organic and aqueous layer. Because caffeine is water-soluble and is a base,sodium carbonate must be added to the hot water to prevent it from reacting with the acids present and to ensure it remains a base. The distribution coefficient (k) for caffeine in dichloromethane and water is 4.6. 3. mixture was transferred after it cooled. Introduction After two extractions with 15mL each, 0.105g of caffeine is in the water and 0.195g is in the dichloromethane. Theoretically, the intermolecular forces of gallic acid can be manipulated to induce a stronger dipole-ion interaction. A vacuum funnel using a Buchner funnel was used to remove the liquid and impurities and the remaining crystals were washed and transferred using a few drops of hexanes. Using the proper extraction methods, the caffeine within a tea bag could potentially be isolated to yield a pure solid; the mass of this solid would reflect the actual yield of caffeine in the tea. A wood stick served as a boiling stick to prevent superheating. Caffeine , Analysis of caffeine SHUBHAMGWAGH 5.7k views Supercritical carbon dioxide extraction of caffeine using green tea leaves nurathirah170 67 views Analytical chemistry report: Analysis of food additives by hplc and uv vis Awad Albalwi 1.7k views Chemical lab report analysis of food additives by hplc and uv-vis Awad Albalwi Carefully press out as much filtrate as possible since the caffeine is in the aqueous layer. Extraction process selectively dissolves one or more of the mixture compounds into a suitable solvent. When weighed, the beaker, stones, and caffeine rendered a total mass of 27.58 g. The difference in mass between the initial weight of the beaker and the final product was then the actual yield of caffeine, 0.02 g. In comparison to the theoretical mass, the experiment provided 18.18% yield of caffeine. Procedure After removing the tea leaves from ten tea bags the tea was weighed and placed in one large tea bag to be boiled for ten minutes in a large beaker filled with ~150 mL of water. Table2. to help you write a unique paper. in a round bottom tannin as an electrophile. Caffeine Extraction from Tea and Coffee January 2017 In book: Comprehensive Organic Chemistry Experiments for the Laboratory Classroom (pp.p. Methylene chloride was added 2 more times to assure that all of the caffeine was reacted with. 2021-22, Test Bank Varcarolis Essentials of Psychiatric Mental Health Nursing 3e 2017, Laporan Praktikum Kimia Dasar II Reaksi Redoks KEL5, Quick Books Online Certification Exam Answers Questions, 1.1 Functions and Continuity full solutions. In this experiment, we aimed to extract caffeine from the tea leaves in the tea bags provided beginning with a solid-liquid extraction method and then a liquid-liquid extraction. an error with the balances, or the ball flask could not have been dried enough after washing. Later this semester, you will be required to make solid derivatives of other compounds. The caffeine can then be extracted from the water by methylene chloride in almost pure form.
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extraction of caffeine from tea lab report 2023